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KMID : 0380620110430020213
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Korean Journal of Food Science and Technology
2011 Volume.43 No. 2 p.213 ~ p.219
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Effect of Eupatorium japonicum Extract on the Metastasis, Invasion and Adhesion of MDA-MB-231 Human Breast Cancer Cells
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Woo Eun-Young
Park So-Young
Kwon Soo-Jin
Kwon Gyoo-Taik
Kim Jong-Dai
Lim Soon-Sung
Yoon Jung-Han
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Abstract
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The metastatic effect of Eupatorium japonicum extract (EJE) on MDA-MB-231 human breast cancer cells was investigated. MDA-MB-231 cells were treated with various concentrations of EJE (0, 5, 10 and 20 μg/mL). EJE inhibited cell migration, invasion and adhesion of MDA-MB-231 cells in dose-dependent manners. Gelatin zymography exhibited that EJE significantly down regulated secretion of matrix metalloproteinase (MMP)-9 and MMP-2. EJE decreased the protein levels of tissue inhibitor of metalloproteinase (TIMP)-1 but increased TIMP-2 levels. Additionally, EJE reduced the protein and mRNA levels of urokinase-type plasminogen activator (uPA), vascular endothelial growth factor (VEGF) and intercellular adhesion molecule (ICAM). In several solvent fractions of EJE, the hexane fraction markedly decreased MDAMB- 231 cell migration. Thus, these finding suggest that EJE may be a potential antimetastatic agent, which can considerably inhibit the metastatic and invasive capacity of breast cancer cells.
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KEYWORD
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Eupatorium japonicum, breast cancer, metastasis, matrix metalloproteinase, urokinase-type plasminogen activator
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